![]() Proteostatic mechanisms are some of the cell’s most essential processes, as they ensure that functional proteins are maintained at their correct concentrations and in the proper locations needed for cellular activities to proceed ( 8, 9, 10). Protein turnover is regulated by several tightly controlled processes that help facilitate protein homoeostasis, also known as proteostasis ( 1, 2, 3, 4, 5, 6, 7). In all cells, proteins are continuously produced and degraded, a process referred to as protein turnover. We highlight important considerations for designing proteome turnover experiments, key biological findings regarding the conserved principles of proteome turnover regulation, and future perspectives for both technological and biological investigation. Here, we outline historical and current approaches to measuring the kinetics of protein turnover on a proteome-wide scale in both steady-state and dynamic systems, with an emphasis on metabolic tracing using stable isotope–labeled amino acids. Thus, probing the kinetics and dynamics of protein turnover lends insight into how cells regulate essential processes such as growth, differentiation, and stress response. ![]() At a steady state, protein turnover is constant to maintain protein homeostasis, but in dynamic responses, proteins change their rates of synthesis and degradation to adjust their proteomes to internal or external stimuli. Collectively, the processes of protein synthesis and degradation are referred to as protein turnover. In all cells, proteins are continuously synthesized and degraded to maintain protein homeostasis and modify gene expression levels in response to stimuli.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |